The answer depends on the questions you’re trying to answer, as well as the origin, quantity, and quality of your samples. In this article, we’ll briefly discuss several RNA-seq workflows, how they ...

RNA plays critical roles in nearly all biological processes, but current methods for sequencing RNA are significantly limited. This is partially because RNA molecules are chemically modified within ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

Selecting an RNA-seq workflow requires more than matching a kit to a sample type. Choices around ribodepletion, strandedness, and unique molecular identifiers (UMIs) directly influence data quality ...

A computational method called scSurv, developed by researchers at Institute of Science Tokyo, links individual cells to ...

Circular RNA (circRNA) is a unique type of non-coding RNA produced by back splicing of mRNA to form the covalent junction without 3’-5’ polarity and poly(A) tail. CircRNA was first discovered about 30 ...

Genome editing using CRISPR/Cas9 "gene scissors" is a powerful tool for biological discovery and for identifying novel drug targets. In pooled CRISPR screens, a large number of cells are edited ...

While RNA sequencing (RNA-seq) has become a standard tool for profiling which genes are active in an organism, determining the actual biological ...